This was not the first time that microbiologists experienced problems with agar. A phenomenon called “The Great Plate Count Anomaly” baffled researchers in the early 20th century when they observed that the number of cells seen under a microscope didn’t match the actual number of colonies growing on an agar plate. Investigating this discrepancy, researchers found agar itself to be the culprit: when nutrient broths are heated with agar during boiling, harmful byproducts (hydroperoxide) can form due to the reaction of agar with phosphate minerals contained in the media. Researchers can avoid this by autoclaving agar separately from the nutrient broth, or by reducing the amount of agar used.
The report pointed out how birth rates in the U.S. have been below the minimum replacement rate since 2008, meaning that the bulk of population growth since then has been the result of immigration. This has proved especially true for the country’s labor force. Nearly 80% of immigrants are of working age, according to the Census, and they account for 19% of the workforce, around 33 million people.
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